Description
Vascular endothelial growth factor C (VEGF-C) is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. The human gene encoding it is VEGFC, and is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGF-C has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans. The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with a molecular mass of 23kDa after glycosylation. ScienCell's human VEGF-C ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human VEGF-C-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human VEGF-C in the sample that is captured on the strips